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Because Listeria is so widespread sources of infections are numerous.

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In outbreaks caused by dairy products, cattle with mastitis may be the source of the organism. The ability to isolate the organism has been impeded in the past by lack of an effective selective medium, as Listeria monocytogenes can be easily and completely overgrown by competing flora.

The medium utilises i the selective inhibitory components lithium chloride, acriflavine, colistin sulphate, cefotetan, cycloheximide or amphotericin B and fosfomycin, ii the indicator system aesculin and ferrous iron for the isolation or differentiation of Listeria monocytogenes. Listeria monocytogenes hydrolyses aesculin, producing black zones around the colonies due to the formation of black iron phenolic compounds derived from the aglucon.

Gram-negative bacteria are completely inhibited.

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Some staphylococci may grow as aesculin-negative colonies. For detection of Listeria monocytogenes when present in small numbers, the test samples must be inoculated into an enrichment broth to allow multiplication before isolation and identification.

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Depending on the type of sample under test, an appropriate method and selective enrichment broth should be chosen prior to inoculation onto the Listeria Selective Medium plates. Appearance Dehydrated medium: Straw-coloured, free-flowing powder Prepared medium: Pale green-coloured gel Quality control.

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Mix well and pour into sterile Petri dishes. Most infections in adult humans are symptomless and result in intestinal, vaginal and cervical carriage.

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Infection during pregnancy may cause abortion, premature delivery and neonatal infection. The possibility of listeriosis should be considered in any woman with unexplained recurrent miscarriage, premature labour or foetal death.

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The most common clinical manifestation in both adults and neonates is meningitis. Widely disseminated infection, abscesses, sub-acute bacterial endocarditis and opportunistic infections in immunosuppressed patients occur less frequently.

Birds, fish and other animals are all susceptible to infection with Listeria. It is of particular importance in domestic farm animals. In the Federal Republic of Germany reporting of listeriosis in animals is compulsory and meat inspection law in the same country requires examination for Listeria because of its significance in meat hygiene.

Listeria monocytogenes is very widespread in the environment.